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yeast gal4 dna binding domain expression vector pas2 1  (TaKaRa)


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    Structured Review

    TaKaRa yeast gal4 dna binding domain expression vector pas2 1
    Yeast Gal4 Dna Binding Domain Expression Vector Pas2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/domain+expression+vector+pas2+1/pmCherry+Vector/pmc03695902-56-6-14
    Average 96 stars, based on 619 article reviews
    yeast gal4 dna binding domain expression vector pas2 1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: Proteomic and functional analysis of NCS-1 binding proteins reveals novel signaling pathways required for inner ear development in zebrafish
    Article Snippet: .. Full length human NCS-1 was subcloned into the yeast GAL4 DNA-binding domain expression vector pAS2-1 (Clontech, Palo Alto, CA), while the human brain cDNA library was subcloned into the GAL4 activation domain vector pACT2 (Clontech). ..

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described ( Lin et al., 2001 ).

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described (Lin et al., 2001).

    cDNA Library Assay:

    Article Title: Proteomic and functional analysis of NCS-1 binding proteins reveals novel signaling pathways required for inner ear development in zebrafish
    Article Snippet: .. Full length human NCS-1 was subcloned into the yeast GAL4 DNA-binding domain expression vector pAS2-1 (Clontech, Palo Alto, CA), while the human brain cDNA library was subcloned into the GAL4 activation domain vector pACT2 (Clontech). ..

    Activation Assay:

    Article Title: Proteomic and functional analysis of NCS-1 binding proteins reveals novel signaling pathways required for inner ear development in zebrafish
    Article Snippet: .. Full length human NCS-1 was subcloned into the yeast GAL4 DNA-binding domain expression vector pAS2-1 (Clontech, Palo Alto, CA), while the human brain cDNA library was subcloned into the GAL4 activation domain vector pACT2 (Clontech). ..

    Plasmid Preparation:

    Article Title: Proteomic and functional analysis of NCS-1 binding proteins reveals novel signaling pathways required for inner ear development in zebrafish
    Article Snippet: .. Full length human NCS-1 was subcloned into the yeast GAL4 DNA-binding domain expression vector pAS2-1 (Clontech, Palo Alto, CA), while the human brain cDNA library was subcloned into the GAL4 activation domain vector pACT2 (Clontech). ..

    Construct:

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described ( Lin et al., 2001 ).

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described (Lin et al., 2001).

    Generated:

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described ( Lin et al., 2001 ).

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described (Lin et al., 2001).

    Subcloning:

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described ( Lin et al., 2001 ).

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described (Lin et al., 2001).

    Polymerase Chain Reaction:

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described ( Lin et al., 2001 ).

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described (Lin et al., 2001).

    Amplification:

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described ( Lin et al., 2001 ).

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described (Lin et al., 2001).

    DNA Sequencing:

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described ( Lin et al., 2001 ).

    Article Title: Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor
    Article Snippet: .. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365–446), D2 (residues 428–443), D3 (residues 385–400), D4 (residues 370–387), and D5 (residues 360–477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2–1 (Clontech, Palo Alto, CA). .. For the D2 receptor screen, the D2–pAS2–1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described (Lin et al., 2001).



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